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Quantification of Strigolactones.

Carlos Rial1, Rosa M Varela1, José M G Molinillo1

  • 1Allelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), School of Sciences, University of Cadiz, Cadiz, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2019
PubMed
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This study presents a new LC-MS/MS method for quantifying strigolactones (SLs), plant hormones crucial for plant development and interactions. The method enables precise measurement of these vital compounds in plant root exudates and extracts.

Area of Science:

  • Plant Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Strigolactones (SLs) are plant hormones regulating shoot branching and influencing symbiotic and parasitic plant interactions.
  • Their roles in the rhizosphere and as plant hormones are areas of active research.
  • Low natural abundance and rapid degradation necessitate sensitive quantification methods.

Purpose of the Study:

  • To develop a rapid and sensitive analytical method for quantifying strigolactones.
  • To establish a protocol for SL detection in plant root exudates and extracts.
  • To facilitate further research into the biological roles of strigolactones.

Main Methods:

  • Development of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) protocol.
Keywords:
Arbuscular mycorrhizal fungiCarotenoidsLC-MS/MSParasitic weedsQuantification methodStrigolactones

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  • Utilized GR24 as an internal standard (IS) for accurate quantification.
  • Applied the method to analyze strigolactones in root exudates and extracts.

    • Main Results:

    • A robust LC-MS/MS method was successfully developed for SL quantification.
    • The method demonstrated high sensitivity, suitable for low-concentration samples.
    • The protocol allows for the analysis of SLs in complex plant matrices.

    Conclusions:

    • The developed LC-MS/MS method provides a crucial tool for strigolactones research.
    • This analytical advancement supports studies on plant hormone functions and rhizosphere interactions.
    • The protocol aids in overcoming challenges associated with low SL abundance and stability.