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Weight Loss Results in Increased Expression of Anti-Inflammatory Protein CRISPLD2 in Mouse Adipose Tissue
Robert M Jackson1, Beth A Griesel1, Kevin R Short2
1Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
Objective:
Obesity is a major risk factor for cardiovascular disease, metabolic syndrome, and type 2 diabetes mellitus, whereas weight loss is associated with improved health outcomes. It is therefore important to learn how adipose contraction during weight loss contributes to improved health. It was hypothesized that adipose tissue undergoing weight loss would have a unique transcriptomic profile, expressing specific genes that might improve health.
Methods:
This study conducted an RNA-sequencing analysis of the epididymal adipose tissue of mice fed either a high-fat diet (HFD) or a regular rodent chow diet (RD) ad libitum for 10 weeks versus a cohort of mice fed HFD for the first 5 weeks before being swapped to an RD for the remainder of the study (swapped diet [SWAP]).
Results:
The swapped diet resulted in weight loss, with a parallel improvement in insulin sensitivity. RNA sequencing revealed several transcriptomic signatures distinct to adipose tissue in SWAP mice, distinguished from both RD and HFD adipose tissue. The analysis found a unique upregulated mRNA that encodes a secreted lipopolysaccharide-binding glycoprotein (CRISPLD2) in adipose tissue. Whereas cellular CRISPLD2 protein levels were unchanged, plasma CRIPSLD2 levels increased in SWAP mice following weight loss and could correlate with insulin sensitivity.
Conclusions:
Taken together, these data demonstrate that CRISPLD2 is a circulating adipokine that may regulate adipocyte remodeling during weight loss.
Insights
Weight loss improves health by altering adipose tissue gene expression. Researchers identified CRISPPLD2 (a secreted glycoprotein) as a key factor in weight loss-induced improvements in insulin sensitivity.
Area of Science:
- Metabolic Research
- Adipose Tissue Biology
- Genomics
Background:
- Obesity is a significant risk factor for cardiovascular disease, metabolic syndrome, and type 2 diabetes.
- Weight loss interventions are associated with improved health outcomes.
- Understanding adipose tissue changes during weight loss is crucial for improving metabolic health.
Purpose of the Study:
- To investigate the transcriptomic profile of adipose tissue during weight loss.
- To identify specific genes expressed in adipose tissue that may contribute to health improvements.
- To explore the role of adipose contraction in weight loss-associated health benefits.
Main Methods:
- RNA sequencing was performed on epididymal adipose tissue from mice.
- Mice were fed either a high-fat diet (HFD) or a regular diet (RD).
- A swapped diet (SWAP) group experienced weight loss by transitioning from HFD to RD.
Main Results:
- The SWAP group exhibited weight loss and improved insulin sensitivity.
- Adipose tissue in SWAP mice showed distinct transcriptomic signatures compared to HFD and RD groups.
- A unique upregulated mRNA encoding secreted lipopolysaccharide-binding glycoprotein (CRISPLD2) was identified; plasma levels correlated with insulin sensitivity.
Conclusions:
- CRISPLD2 is identified as a circulating adipokine.
- CRISPLD2 may play a role in regulating adipocyte remodeling during weight loss.
- These findings highlight CRISPLD2 as a potential mediator of weight loss benefits.
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