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Updated: Jan 3, 2026
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
Light-driven visualization of endogenous cysteine, homocysteine, and glutathione using a near-infrared fluorescent
Yang Yang1, Yingzhe Wang, Yan Feng
1Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. liuws@lzu.edu.cn.
Abstract:
Herein, we presented a hydrosoluble triple-site and triple-excitation alternative NIR fluorescent probe for visualization of endogenous biothiols in phosphate-buffered saline (pH 7.4, 10 mM). Upon irradiation using different excitation light, probe 1 exhibited different fluorescence responses upon the addition of Cys, Hcy, and GSH: λex = 419 nm, λem = 498 nm; λex = 518 nm, λem = 573, 616, 727, and 783 nm; λex = 555 nm, λem = 612 and 727 nm, respectively. Furthermore, 1 was favourably applied for bioimaging endogenous Cys, Hcy, and GSH in A375 cells through well-defined blue-green-red emission channels.
