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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
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High-Throughput Solid-Phase Building Block Synthesis for DNA-Encoded Libraries.

Claudio Zambaldo1, Stefanie N Geigle1, Alexander L Satz1

  • 1Roche Innovation Center , Basel 4070 , Switzerland.

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|November 21, 2019
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Summary
This summary is machine-generated.

This study introduces a cost-effective method for synthesizing thousands of diverse molecular building blocks (BBs) using DNA-conjugation for encoding, purification, and analysis. This approach enables high-throughput generation of sp3-rich compounds for various applications.

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Area of Science:

  • Organic Chemistry
  • Synthetic Chemistry
  • Bioconjugation Chemistry

Background:

  • Solid-phase synthesis enables the production of large numbers of crude products.
  • DNA-conjugation offers unique capabilities for molecular encoding and analysis.

Purpose of the Study:

  • To develop a generalizable and cost-effective method for synthesizing diverse molecular building blocks (BBs).
  • To demonstrate a practical application of crude reaction mixtures using DNA-conjugation.
  • To enable high-throughput generation and analysis of sp3-rich BBs.

Main Methods:

  • Employing DNA-incompatible chemistries for BB synthesis.
  • Utilizing solid-phase synthesis to generate crude reaction mixtures.
  • Applying DNA-conjugation for simultaneous encoding, purification, and analysis of products.

Main Results:

  • Successful synthesis of thousands of diverse BBs.
  • Demonstrated practical utility of crude synthesis mixtures.
  • Generated sp3-rich BBs that were successfully encoded by DNA.
  • Enabled rapid analysis of synthesized products.

Conclusions:

  • The developed workflow provides a generalizable and cost-effective approach for synthesizing a large number of BBs.
  • DNA-conjugation is a powerful tool for encoding, purifying, and analyzing products from crude synthesis mixtures.
  • This method facilitates high-throughput generation of valuable sp3-rich building blocks.