Related Experiment Video

Updated: Jan 3, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.6K

Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific

Arie J Hoogendijk1, Farzin Pourfarzad2, Cathelijn E M Aarts2

  • 1Department of Molecular and Cellular Hemostasis, Sanquin Research, Amsterdam, the Netherlands.

Cell Reports
|November 21, 2019
PubMed

Insights

Human neutrophil development involves dynamic changes from progenitor cells to mature immune cells. This study reveals how RNA and protein interactions drive functional maturation for host defense.

Area of Science:

  • Immunology
  • Cell Biology
  • Proteomics

Background:

  • Human neutrophilic granulocytes are key innate immune cells essential for combating bacterial and fungal infections.
  • The developmental transition of myeloid progenitor cells into mature, non-dividing neutrophils is complex and not fully understood.

Purpose of the Study:

  • To investigate the dynamic molecular changes during human neutrophil development.
  • To integrate proteomic and transcriptomic data to understand gene regulation during neutrophil maturation.

Main Methods:

  • Utilized mass spectrometry-based quantitative proteomics.
  • Employed transcriptomic data analysis.
  • Examined five distinct developmental stages of neutrophils in bone marrow and blood.

Main Results:

  • Identified dynamic changes in protein and RNA levels throughout neutrophil development.
  • Revealed complex interactions between RNA and protein kinetics.
  • Linked these dynamic changes to the functional maturation of neutrophil killing activities.

Conclusions:

  • Human neutrophil development is characterized by highly dynamic and coordinated RNA-protein interactions.
  • These molecular dynamics are crucial for the acquisition of mature immune effector functions in neutrophils.
  • This study provides a comprehensive view of neutrophil differentiation at the proteome and transcriptome levels.
Keywords:
granule proteinsgranulopoiesisneutrophil developmentproteomicstranscriptomics

More Related Videos

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

990
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

12.7K

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.8K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.6K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
15.7K