Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

59.3K
Overview
59.3K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

1.1K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.1K
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

6.7K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
6.7K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

736
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
736
Antimicrobial Proteins01:23

Antimicrobial Proteins

12.8K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
12.8K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

441
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
441

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cannabinoid (CB) 1 receptor antagonists block fentanyl conditioned place preference but not fentanyl antinociception.

Drug and alcohol dependence·2026
Same author

Library Docking for Cannabinoid-2 Receptor Ligands.

Journal of medicinal chemistry·2026
Same author

METTL3 regulates exocytosis independently of m<sup>6</sup>A.

Science advances·2026
Same author

Mitochondrial dysfunction in muscle cells induced by snoring vibrations.

Mitochondrion·2026
Same author

Identification of drug repurposing candidates for the treatment of polycystic kidney disease.

British journal of pharmacology·2026
Same author

A deformylase inhibitor expands therapeutic options for Lyme disease.

Research square·2026

Related Experiment Video

Updated: Jan 3, 2026

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
11:56

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

14.6K

A new antibiotic selectively kills Gram-negative pathogens.

Yu Imai1, Kirsten J Meyer1, Akira Iinishi1

  • 1Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, MA, USA.

Nature
|November 21, 2019
PubMed
Summary

Researchers discovered darobactin, a novel antibiotic effective against drug-resistant Gram-negative pathogens. This compound, derived from bacterial symbionts, targets essential outer membrane proteins, offering a promising new therapeutic avenue.

More Related Videos

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.2K
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

12.9K

Related Experiment Videos

Last Updated: Jan 3, 2026

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
11:56

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

14.6K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.2K
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

12.9K

Area of Science:

  • Microbiology
  • Drug Discovery
  • Biochemistry

Background:

  • The rise of drug-resistant Gram-negative pathogens necessitates novel antibiotics.
  • Gram-negative bacteria possess a restrictive permeability barrier hindering antibiotic penetration.
  • The development of new antibiotic classes against Gram-negative bacteria has stalled since the 1960s.

Purpose of the Study:

  • To identify novel antibiotic compounds effective against drug-resistant Gram-negative pathogens.
  • To explore bacterial symbionts as a source for new antimicrobial agents.
  • To characterize a newly discovered antibiotic, darobactin.

Main Methods:

  • Screening of Photorhabdus bacterial isolates for antibiotic activity.
  • Investigating the genetic basis and biosynthesis of darobactin.
  • Evaluating darobactin's efficacy in vitro and in animal infection models.
  • Analyzing the mechanism of resistance by identifying resistant mutants.

Main Results:

  • Discovery of darobactin, a novel ribosomally synthesized antibiotic.
  • Darobactin exhibits potent activity against Gram-negative pathogens in vitro and in vivo.
  • Darobactin possesses a unique structure with two post-translationally formed fused rings.
  • Resistance to darobactin arises from mutations in BamA, an essential outer membrane protein translocator.

Conclusions:

  • Bacterial animal symbionts harbor promising antibiotic candidates.
  • Darobactin represents a potential therapeutic agent for treating Gram-negative bacterial infections.
  • Targeting BamA offers a viable strategy for developing new antibiotics against challenging pathogens.