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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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Related Experiment Video

Updated: Jan 3, 2026

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
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Optimal Timing of Repeat Multiplex Molecular Testing for Respiratory Viruses.

Yamini Mandelia1, Gary W Procop2, Sandra S Richter2

  • 1Center for Pediatric Infectious Diseases, Cleveland Clinic, Cleveland, Ohio, USA mandely@ccf.org esperf@ccf.org.

Journal of Clinical Microbiology
|November 22, 2019
PubMed
Summary

Repeat multiplex nucleic acid amplification tests (NAATs) for respiratory viruses within 20 days show minimal result changes. This finding supports reducing unnecessary repeat testing to improve laboratory stewardship and cut costs.

Keywords:
NAATcommunity-acquired pneumoniainfluenzalaboratory stewardshipmultiplex PCRpneumoniarepeat testingrespiratory syncytial virusrespiratory viral panelrespiratory viruses

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Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Laboratory Medicine

Background:

  • Multiplex nucleic acid amplification tests (NAATs) are crucial for diagnosing respiratory viruses.
  • Determining optimal timing for repeat testing is challenging, impacting resource allocation and patient care.

Purpose of the Study:

  • To analyze repeat multiplex NAAT results for respiratory viruses within 90 days.
  • To identify the time window for significant changes in test results.
  • To inform laboratory stewardship practices regarding respiratory viral testing.

Main Methods:

  • Analysis of 5 years of multiplex NAAT results for 14 respiratory viruses.
  • Inclusion of repeat nasopharyngeal specimens tested within 90 days of initial testing.
  • Logistic regression to compare odds of result change across different repeat testing intervals.

Main Results:

  • 12% of specimens were repeat tests within 90 days; 71% showed no result change.
  • Initially positive results remained positive for 11-15 days; negative results generally stayed negative.
  • Odds of result change plateaued after 20 days, with significantly lower odds for repeats within 20 days.

Conclusions:

  • Repeat multiplex NAATs within 20 days often yield redundant results, increasing costs.
  • Evidence supports limiting repeat respiratory viral testing to before 20 days to avoid unnecessary procedures.
  • Findings provide a basis for laboratory stewardship to reduce unwarranted respiratory viral testing.