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Self-replenishment cycles generate a threshold response.

Hiroyuki Kurata^1,2

¹Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Fukuoka, Japan. kurata@bio.kyutech.ac.jp.

Scientific Reports

|November 22, 2019

Summary

This summary is machine-generated.

This study defines novel self-replenishment metabolic cycles, distinct from elementary cycles. These cycles exhibit a threshold response, acting like digital switches to control metabolic status.

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Area of Science:

Biochemistry
Systems Biology
Metabolic Engineering

Background:

Metabolic cycles are crucial for cellular energy and mass conversion.
Existing models often overlook self-replenishment cycles.
Understanding cycle stoichiometry is key to metabolic control.

Purpose of the Study:

To define and characterize self-replenishment metabolic cycles.
To elucidate the design principles governing these cycles.
To investigate their functional importance and regulatory mechanisms.

Main Methods:

Stoichiometric analysis to classify metabolic cycles.
Theoretical modeling to derive the design principle of self-replenishment cycles.
Kinetic simulations of self-replenishment cycles in E. coli.

Main Results:

Two types of metabolic cycles identified: elementary and self-replenishment.
Self-replenishment cycles autonomously supply substrates internally.
Theoretical analysis revealed a threshold response mechanism.
Simulations demonstrated digital switch-like metabolic control.

Conclusions:

Self-replenishment cycles represent a functionally important, previously overlooked class of metabolic networks.
These cycles operate via a threshold response, enabling precise metabolic control.
The identified design principles offer insights into metabolic engineering and systems biology.