Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

9.8K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systematic evaluation of BBB-penetrant AAV capsids in marmosets identifies VCAP-102 as a highly efficient brain-transducing capsid.

Molecular therapy. Advances·2026
Same author

Inducible rAAV producer cell lines yield vectors equivalent to transient transfection: a physicochemical and biological comparison.

Scientific reports·2026
Same author

A compact GAD67 promoter enables inhibitory neuron-targeted AAV gene therapy for seizure suppression.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Poor sleep quality correlates with axial symptoms and mood problems in Parkinson's disease.

Dialogues in clinical neuroscience·2026
Same author

Aromatic-Turmerone Analogs Activate Chaperone-Mediated Autophagy and Ameliorate Dendritic Shrinkage in Purkinje Cell Models of Spinocerebellar Ataxia.

Biological & pharmaceutical bulletin·2026
Same author

Establishment of spinocerebellar ataxia type 34 model mice accompanied by early glial activation and degeneration of cerebellar neurons.

Journal of pharmacological sciences·2026

Related Experiment Video

Updated: Jan 3, 2026

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
07:35

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Published on: March 27, 2021

13.2K

Advanced CUBIC tissue clearing for whole-organ cell profiling.

Katsuhiko Matsumoto1, Tomoki T Mitani1,2,3, Shuhei A Horiguchi1,4

  • 1Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka, Japan.

Nature Protocols
|November 22, 2019
PubMed
Summary

Advanced tissue clearing methods enable rapid, high-resolution imaging of whole organs. The Clear, Unobstructed Brain Imaging Cocktails and Computational Analysis (CUBIC) protocol accelerates biological research and pathological diagnosis with efficient data processing.

More Related Videos

Optical Clearing and Imaging of Immunolabeled Kidney Tissue
06:18

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

10.9K
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
14:19

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Published on: March 25, 2022

4.3K

Related Experiment Videos

Last Updated: Jan 3, 2026

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
07:35

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Published on: March 27, 2021

13.2K
Optical Clearing and Imaging of Immunolabeled Kidney Tissue
06:18

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

10.9K
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
14:19

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Published on: March 25, 2022

4.3K

Area of Science:

  • Biological imaging
  • Biomedical research
  • Pathological diagnosis

Background:

  • Tissue-clearing techniques are essential for detailed biological research and diagnostics.
  • Existing methods can be time-consuming and may compromise signal integrity.

Purpose of the Study:

  • To introduce an advanced protocol for rapid, high-transparency organ preparation and imaging.
  • To enable efficient computational analysis of large-scale 3D imaging data.

Main Methods:

  • Immersion of organs in Clear, Unobstructed Brain Imaging Cocktails and Computational Analysis (CUBIC) reagents for 7-21 days.
  • High-speed imaging systems capable of processing >0.5 TB/h/color.
  • Computational analysis for extracting cell positions from large datasets (2.5-14 TB) within 3-12 hours.

Main Results:

  • Achieved high-transparency organs while preserving fluorescent protein signals.
  • Demonstrated efficient data processing, extracting cell data from large datasets rapidly.
  • Successfully applied the protocol to quantify cells in whole mouse brains, including AAV-transduced cells.

Conclusions:

  • The CUBIC protocol significantly enhances throughput for analyzing numerous biological samples.
  • This method provides a powerful platform for next-generation biomedical research and diagnostics.
  • Enables detailed analysis of cellular distribution and gene transduction in whole organs.