Physiological response of Secale cereale L. seedlings under freezing-thawing and alkaline salt stress

Freezing-thawing and saline-alkaline are the major abiotic stress for the pasture in most high-latitude areas, which are serious threats to the yield of pasture. In this study, the osmotic adjustment substances, membrane lipid peroxidation, and antioxidant enzymes activities of rye (Secale cereale L., cv. Dongmu-70) seedlings under different treatments: CK (no treatment), SC (Na₂CO₃ treatment), FT (freezing-thawing treatment), and FT+SC (combined Na₂CO₃ and freezing-thawing treatments), were investigated. At the freezing stage, the content of MDA and proline, the activity of APX, SOD, and POD increased with the decrease of the temperature in the leaves of rye seedlings in FT and FT+SC treatments and reached the maximum value at - 5 °C. In addition, the content of protein and H₂O₂, CAT activity reached the maximum value at 0 °C; the damage is larger under low temperature stress at 0 °C and - 5 °C in rye seedling. At the thawing stage, the content of MDA and H₂O₂ in seedling leaves decreased in FT and FT + SC treatments. These results demonstrated that proline content and antioxidant enzymes activities could play an important role in protecting cytomembrane and scavenging ROS respectively in rye under alkaline salt stress and freezing-thawing stress. The result also indicated rye seedlings were subjected to a freezing-thawing stress which resulted in a reversible (recoverable) injury.