Related Experiment Video

Updated: Jan 3, 2026

Transarterial Administration of Oncolytic Viruses for Locoregional Therapy of Orthotopic HCC in Rats
08:55

Transarterial Administration of Oncolytic Viruses for Locoregional Therapy of Orthotopic HCC in Rats

Published on: April 15, 2016

9.9K

A novel oncolytic adenovirus inhibits hepatocellular carcinoma growth

Yu-Huan Bai1,2, Xiao-Jing Yun2, Yan Xue2

  • 1Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, China.

Journal of Zhejiang University. Science. B
|November 22, 2019
PubMed
Abstract

Insights

A novel oncolytic adenovirus, GP73-SphK1sR-Ad5, effectively inhibits hepatocellular carcinoma (HCC) growth by downregulating SphK1. This targeted therapy shows high specificity and efficacy in preclinical models.

Area of Science:

  • Oncolytic virotherapy
  • Hepatocellular carcinoma (HCC) research
  • Gene therapy targeting cancer

Background:

  • Hepatocellular carcinoma (HCC) remains a significant global health challenge.
  • Targeted therapies are crucial for improving HCC treatment outcomes.
  • Oncolytic adenoviruses (OAs) represent a promising strategy for cancer treatment.

Purpose of the Study:

  • To evaluate the inhibitory potential of a novel oncolytic adenovirus, GP73-SphK1sR-Ad5, against hepatocellular carcinoma (HCC) growth.
  • To assess the specificity and efficacy of GP73-SphK1sR-Ad5 in preclinical HCC models.

Main Methods:

  • Construction of GP73-SphK1sR-Ad5 by integrating GP73 promoter and SphK1-shRNA into Ad5.
  • In vitro studies using HCC Huh7 cells and normal HL-7702 cells to assess gene expression, cell viability (MTT assay), and apoptosis (flow cytometry).
  • In vivo studies using an HCC xenograft mouse model with intratumoral injection of GP73-SphK1sR-Ad5, followed by tumor analysis (volume, weight, survival, histopathology).

Main Results:

  • GP73-SphK1sR-Ad5 specifically upregulated E1A and downregulated SphK1 in HCC cells, not normal liver cells.
  • Significant reduction in HCC cell viability and increased apoptosis were observed post-transfection.
  • Intratumoral administration in mice led to decreased tumor volume and weight, prolonged survival, reduced tumor vascularization, and induced cancer cell death.

Conclusions:

  • GP73-SphK1sR-Ad5 demonstrates significant potential as a novel oncolytic adenovirus for HCC treatment.
  • The agent exhibits high specificity and efficacy in inhibiting HCC progression.
  • Further investigation into GP73-SphK1sR-Ad5 as a targeted therapy for HCC is warranted.
Keywords:
Hepatocellular carcinoma; Oncolytic adenovirus; Golgi protein 73; Sphingosine kinase 1

More Related Videos

Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses06:13

Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses

Published on: November 24, 2014

9.8K
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors19:02

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors

Published on: March 22, 2011

13.8K

Related Concept Videos

Cancer Therapies02:49

Cancer Therapies

Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
9.7K
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
6.8K
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
1.7K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.5K