Extraction process optimization of Murraya koenigii leaf extracts and antioxidant properties

Area of Science:

• Phytochemistry
• Natural Product Chemistry
• Process Optimization

Background:

• Murraya koenigii (curry leaf) is a valuable plant source of bioactive compounds.
• Optimizing extraction is crucial for maximizing the yield and efficacy of plant-derived compounds.
• Phenolic content and antioxidant properties are key indicators of M. koenigii's potential benefits.

Purpose of the Study:

• To optimize extraction parameters (solvent concentration, time) for Murraya koenigii leaf extract.
• To maximize the yield and total phenolic content of the extract.
• To evaluate the antioxidant potential (DPPH scavenging, ferric reducing activity) and flavonoid content of optimized extracts.

Main Methods:

• Response Surface Methodology (RSM) with a Central Composite Design (CCD) was employed.
• Independent variables included solvent type (acetone, ethanol, methanol) at concentrations (20-80%) and extraction time (20-100 min).
• Optimized extracts were analyzed for DPPH scavenging activity, total phenolic content, flavonoid content, and ferric reducing activity.

Main Results:

• Solvent concentration and extraction time significantly influenced extract yield and total phenolic content.
• Optimal conditions identified were 50% ethanol, 60% acetone, and 80% methanol.
• The 50% ethanol extract exhibited the highest DPPH scavenging activity and total phenolic content.
• The 60% acetone extract showed the highest ferric reducing activity and flavonoid content.

Conclusions:

• Optimized extraction using RSM effectively maximized bioactive compound yield from Murraya koenigii leaves.
• Different solvents yield extracts with varying profiles of antioxidant and flavonoid content.
• Ethanol and acetone show promise for extracting specific high-value compounds from M. koenigii.