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Published on: August 23, 2019
MicroRNA-96-3p promotes metastasis of papillary thyroid cancer through targeting SDHB
Xupeng Zhao1, Yingjie Li2, Yong Zhou1
11Department of Fourth General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032 China.
Background:
MicroRNA (MiRNA) is a small non-coding RNA which is implicated in a cohort of biological function in cancer, including proliferation, metastasis, apoptosis and invasion. MiR-96 has been reported to be involved in many cancers, including papillary thyroid cancer. However, the role of miR-96-3p in papillary thyroid cancer metastasis is still unclear.
Methods:
qRT-PCR is used to detect the level of miR-96-3p and mRNA of SDHB in PTC tissues and cell lines. Western blot assays are used to verify the protein expression of SDHB. The transwell assays are performed to identify the migration ability of PTC cell lines. Moreover, dual-luciferase 3'-UTR reporter assays are chosen to illuminate the direct target of miR-96-3p.
Results:
The relative miR-96-3p upregulate in PTC tissues and three PTC cell lines (B-CPAP, K-1 and TPC-1 cells) while the relative SDHB is opposite. Our results revealed that the miR-96-3p promotes metastasis and invasion in PTC cell lines (K-1 and TPC-1 cells) by direct targeting SDHB and influence the downstream protein AKT.
Conclusions:
Taken together, the miR-96-3p is involved in PTC metastasis and invasion by direct targeting SDHB and the downstream molecule AKT and mTOR.
Insights
MicroRNA-96-3p (miR-96-3p) promotes papillary thyroid cancer metastasis by targeting SDHB. This microRNA (miRNA) plays a key role in cancer progression and invasion.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression.
- MiRNAs are implicated in various cancer biological functions, including metastasis and invasion.
- MiR-96 has been linked to several cancers, but its specific role in papillary thyroid cancer (PTC) metastasis remains unclear.
Purpose of the Study:
- To investigate the role of miR-96-3p in papillary thyroid cancer (PTC) metastasis.
- To identify the molecular targets and pathways regulated by miR-96-3p in PTC.
Main Methods:
- Quantitative reverse transcription PCR (qRT-PCR) to measure miR-96-3p and SDHB mRNA levels.
- Western blot assays to assess SDHB protein expression.
- Transwell assays to evaluate cell migration and invasion.
- Dual-luciferase reporter assays to confirm direct targeting of SDHB by miR-96-3p.
Main Results:
- MiR-96-3p was upregulated in PTC tissues and cell lines.
- SDHB expression was inversely correlated with miR-96-3p levels.
- MiR-96-3p promoted metastasis and invasion in PTC cell lines (K-1 and TPC-1).
- MiR-96-3p directly targets SDHB, influencing downstream AKT signaling.
Conclusions:
- MiR-96-3p is significantly involved in PTC metastasis and invasion.
- The mechanism involves direct targeting of SDHB and downstream regulation of AKT and mTOR pathways.
- MiR-96-3p represents a potential therapeutic target for papillary thyroid cancer.
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