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Updated: Jan 3, 2026
Diffusion Tensor Magnetic Resonance Imaging in the Analysis of Neurodegenerative Diseases
Published on: July 28, 2013
Cerebral degeneration in amyotrophic lateral sclerosis: A prospective multicenter magnetic resonance spectroscopy
Ojas Srivastava1, Chris Hanstock1, Sneha Chenji1
1Faculty of Science (OS); Department of Biomedical Engineering (CH, PS, SK); Neuroscience and Mental Health Institute (SC, DT, SK); Divison of Neurology (DM, CL, SK), Department of Medicine; School of Public Health (DE); University of Alberta, Edmonton, Alberta; Sunnybrook Health Sciences Centre (LZ, AA, SJG), University of Toronto, Toronto, Ontario; Montreal Neurological Institute and Hospital (AG), McGill University, Montreal, Quebec; Departments of Radiology and Clinical Neurosciences (LK, RF), Hotchkiss Brain Institute, University of Calgary; and Seaman Family MR Research Centre (LK, RF), Foothills Medical Centre, Calgary, Alberta, Canada.
Background:
We investigated cerebral degeneration and neurochemistry in patients with amyotrophic lateral sclerosis (ALS) using magnetic resonance spectroscopy (MRS).
Methods:
We prospectively studied 65 patients and 43 age-matched healthy controls. Participants were recruited from 4 centers as part of a study in the Canadian ALS Neuroimaging Consortium. All participants underwent single-voxel proton MRS using a protocol standardized across all sites. Metabolites reflecting neuronal integrity (total N-acetyl aspartyl moieties [tNAA]) and gliosis (myo-inositol [Ino]), as well as creatine (Cr) and choline (Cho), were quantified in the midline motor cortex and midline prefrontal cortex. Comparisons were made between patients with ALS and healthy controls. Metabolites were correlated with clinical measures of upper motor neuron dysfunction, disease progression rate, and cognitive performance.
Results:
In the motor cortex, tNAA/Cr, tNAA/Cho, and tNAA/Ino ratios were reduced in the ALS group compared with controls. Group differences in tNAA/Cr and tNAA/Cho in the prefrontal cortex displayed reduced ratios in ALS patients; however, these were not statistically significant. Reduced motor cortex ratios were associated with slower foot tapping rate, whereas only motor tNAA/Ino was associated with finger tapping rate. Disease progression rate was associated with motor tNAA/Cho. Verbal fluency, semantic fluency, and digit span forwards and backwards were associated with prefrontal tNAA/Cr.
Conclusions:
This study demonstrates that cerebral degeneration in ALS is more pronounced in the motor than prefrontal cortex, that multicenter MRS studies are feasible, and that motor tNAA/Ino shows promise as a potential biomarker.
