Nitrincola tapanii sp. nov., a novel alkaliphilic bacterium from An Indian Soda Lake

A novel Gram-stain-negative bacterial strain designated as MEB193^T was isolated from a sediment sample collected from Lonar Lake, India. The cells were motile, non-spore-forming and rod-shaped. The strain was oxidase- and catalase-positive. It grew optimally at pH 9.0 and at 1 % (w/v) NaCl concentration at 30 °C. Based on 16S rRNA gene sequence similarity, MEB193^T belongs to genus Nitrincola, with Nitrincola alkalilacustris ZV-19^T (95.89 %) and Nitrincola lacisaponensis 4CA^T (95.87 %) as its closest neighbours. The major fatty acid was summed feature 8 comprising C_18:1ω7c/C_18:1ω6c (52 %) followed by C_16 : 0 (25 %). Phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG) were present as the major polar lipids. The draft genome obtained in this study was 2 793 747 bp and the G+C content was 50.79 mol%. Average nucleotide identity (71.76 %) and DNA-DNA hybridization (<20 %) values between strain MEB193^T and Nitrincola lacisaponensis 4CA^T confirmed the novelty of this new species. Based on phenotypic including chemotaxonomic and genotypic characterization data, strain MEB193^T represents a new species of the genus Nitrincola for which the name Nitrincola tapanii sp. nov. is proposed. The type strain is MEB193^T (=MCC 2863^T=JCM 31570 ^T=KCTC 52390 ^T).