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Protein Isolation from the Developing Embryonic Mouse Heart Valve Region
Published on: September 23, 2014
Verification of hub genes in the expression profile of aortic dissection
Weitie Wang1, Qing Liu2, Yong Wang1
1Department of Cardiovascular Surgery, Second Hospital of Jilin University, Changchun, Jilin, China.
Background:
To assess the mRNA expression profile and explore the hub mRNAs and potential molecular mechanisms in the pathogenesis of human thoracic aortic dissection (TAD).
Methodology:
mRNA microarray expression signatures of TAD tissues (n = 6) and non-TAD tissues (NT; n = 6) were analyzed by an Arraystar human mRNA microarray. Real-time PCR (qRT-PCR) was used to validate the results of the mRNA microarray. Bioinformatic tools, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, were utilized. Protein-protein interaction (PPI) networks were constructed based on data from the STRING database. Molecular Complex Detection (MCODE) and cytoHubba analyses were used to predict the strongest hub gene and pathway.
Results:
The top 10 hub genes were CDK1, CDC20, CCNB2, CCNB1, MAD2L1, AURKA, C3AR1, NCAPG, CXCL12 and ASPM, which were identified from the PPI network. Module analysis revealed that TAD was associated with the cell cycle, oocyte meiosis, the p53 signaling pathway, and progesterone-mediated oocyte maturation. The qRT-PCR results showed that the expression of all hub genes was significantly increased in TAD samples (p < 0.05). Immunostaining of Ki-67 and CDK1 showed a high proliferation state and high expression in TAD, respectively.
Conclusions:
CDK1 could be used as a potential diagnostic biomarker and therapeutic target of TAD.
Insights
This study identified key genes involved in thoracic aortic dissection (TAD) pathogenesis. Cyclin-dependent kinase 1 (CDK1) showed high expression and may serve as a diagnostic biomarker and therapeutic target for TAD.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- Thoracic aortic dissection (TAD) is a life-threatening condition.
- Understanding the molecular mechanisms underlying TAD is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the mRNA expression profile in human thoracic aortic dissection (TAD).
- To identify hub messenger RNAs (mRNAs) and elucidate potential molecular mechanisms in TAD pathogenesis.
- To explore CDK1 as a potential diagnostic biomarker and therapeutic target for TAD.
Main Methods:
- mRNA microarray analysis of TAD and non-TAD tissues.
- Validation using quantitative real-time PCR (qRT-PCR).
- Bioinformatic analyses including Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathways, and protein-protein interaction (PPI) network construction.
Main Results:
- Ten hub genes, including CDK1, were identified, with significantly increased expression in TAD samples.
- TAD pathogenesis is associated with cell cycle, oocyte meiosis, and p53 signaling pathways.
- High expression of CDK1 and Ki-67 in TAD tissues indicates a high proliferation state.
Conclusions:
- CDK1 is a significantly upregulated hub gene in TAD.
- CDK1 demonstrates potential as a diagnostic biomarker for TAD.
- CDK1 represents a promising therapeutic target for managing TAD.
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