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Modulation of Cellular Reactivity for Enhanced Cell-Based Biosensing.

Mihaela Gheorghiu1,2, Luciana Stanica1,2, Cristina Polonschii1

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This study introduces a novel biosensing method using optogenetics and impedance assays for rapid, sensitive detection of cellular changes from bioactive compounds. The platform enhances sensitivity and reduces assay time for improved cell-based sensing applications.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Biosensing

Background:

  • Cell-based sensing platforms offer functional insights into compound effects but face challenges in assay duration, reproducibility, and sensitivity.
  • Existing methods often require extended incubation periods and struggle with detecting subtle cellular alterations.

Purpose of the Study:

  • To develop a rapid, highly sensitive biosensing method for quantifying cellular responses to bioactive or toxic compounds.
  • To overcome limitations of current cell-based assays regarding time, sensitivity, and reproducibility.

Main Methods:

  • Integration of optogenetic control in non-electrogenic human cells expressing light-sensitive protein channels.
  • Utilizing a non-invasive electro-optical analytical platform for quantitative assessment of stimulus-dependent cellular responses.
  • Combining optogenetic stimulation with time-lapse fast impedance assays under static and flow conditions.

Main Results:

  • Demonstrated exquisite sensitivity in detecting and quantifying cellular alterations induced by low compound levels.
  • Achieved a significantly reduced assay time compared to conventional methods.
  • Showcased enhanced platform sensitivity through the synergistic interplay of optogenetic stimulation and impedance assays.

Conclusions:

  • The proposed optogenetically modulated cell-based sensing platform offers a new paradigm for impedance-based sensing.
  • The system provides a rapid and sensitive approach suitable for in-field applications.
  • This method significantly improves the assessment of cellular effects from bioactive/toxic compounds.