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Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: Jan 3, 2026

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Single-Particle Tracking with Scattering-Based Optical Microscopy.

Zhongju Ye¹, Xin Wang¹, Lehui Xiao¹

¹State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry , Nankai University , Tianjin , 300071 , China.

Analytical Chemistry

|November 22, 2019

Summary

This summary is machine-generated.

Single-particle tracking (SPT) offers detailed insights into dynamic processes, unlike traditional methods. This Feature explores optical microscopy techniques and data analysis for scattering-based SPT within cells.

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Area of Science:

Biophysics
Cell Biology
Optical Microscopy

Background:

Traditional ensemble measurements obscure individual dynamic behaviors.
Single-particle tracking (SPT) provides high-resolution data on molecular dynamics.
Scattering-based SPT is a key technique for visualizing cellular processes.

Discussion:

This Feature reviews optical microscopic techniques for scattering-based SPT.
It details essential data analysis methods for interpreting SPT data.
The review highlights the utility of SPT in complex cellular environments.

Key Insights:

SPT reveals the distribution of dynamic processes at the single-object level.
Scattering-based SPT offers a powerful lens into cellular mechanics.
Understanding individual particle behavior is crucial for cell biology.

Outlook:

Future advancements in optical microscopy will enhance SPT capabilities.
Refined data analysis will unlock deeper biological insights from SPT.
SPT applications in cell biology are expected to expand significantly.