Structural characterization and anticomplement activity of an acidic polysaccharide from Hedyotis diffusa.
Jiangyan Huo1, Yan Lu2, Yukun Jiao3
1State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China; School of Pharmacy, Fudan University, Shanghai, China.
International Journal of Biological Macromolecules
|November 22, 2019
Summary
An acidic polysaccharide from Hedyotis diffusa exhibits potent anticomplement activity, crucial for immune response modulation. Its uronic acid content is vital for this function, interacting with complement factors C3 and C4.
Area of Science:
- Phytochemistry
- Immunology
- Biochemistry
Background:
- Hedyotis diffusa (Willd.) Roxb. is a medicinal plant with potential therapeutic properties.
- Polysaccharides are known to modulate immune responses.
- The anticomplementary activity of plant-derived polysaccharides is of significant interest.
Purpose of the Study:
- To isolate and characterize polysaccharides from Hedyotis diffusa.
- To evaluate the anticomplement and antioxidant activities of the isolated polysaccharides.
- To investigate the structural basis for the anticomplement activity.
Main Methods:
- Purification of homogeneous polysaccharides (HD-PS-1 and HD-PS-2) using chromatographic techniques.
- Detailed structural elucidation of HD-PS-1 using advanced analytical methods.
- Assay of anticomplement activity (CH50 and AP50) and antioxidant activity (DPPH radical scavenging).
Main Results:
- An acidic polysaccharide (HD-PS-1) with a complex structure including glucuronic acid was isolated.
- HD-PS-1 demonstrated significant anticomplement activity, which was reduced upon modification.
- A neutral polysaccharide (HD-PS-2) showed antioxidant activity but lacked anticomplement activity.
- HD-PS-1 was found to interact with complement components C3 and C4.
Conclusions:
- HD-PS-1 possesses potent anticomplement activity, suggesting its potential as an immunomodulatory agent.
- The presence of uronic acids in HD-PS-1 is essential for its anticomplement activity.
- HD-PS-2 exhibits antioxidant properties, indicating diverse biological activities of Hedyotis diffusa polysaccharides.
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