Microwave assisted extraction and characterization of polysaccharide from waste jamun fruit seeds

The intention of current work is to recover/extract polysaccharide from jamun fruit seeds (waste) by microwave assisted solid-liquid extraction (MAE) technique using four-factor (microwave power (MWP), pH, time (MWT) and solid to liquid (SL) ratio) five-level central composite rotatable experimental design (CCRED). The observed data was evaluated by statistically and a mathematical model (polynomial) was developed to predict the polysaccharide yield. Numerical optimization technique is used to attain the ideal optimal condition (microwave power of 515 w, pH of 3.2, MWT of 3.1 min and SL ratio of 1:15 g/ml) to retrieve maximal yield of polysaccharide and attained optimal condition was experimentally validated. The experimental yield of polysaccharide (4.71 ± 0.02%) was matched with predicted value (4.72%). Physico-chemical properties of the polysaccharide extracted at optimal condition was investigated. Fourier Transform Infrared Spectroscopy (FTIR) evaluation were carried out in this study to characterize the polysaccharide. Scanning Electron Microscopy (SEM) was also utilized to reveal the morphology of raw and extracted plant sample.