Fucoidan from sea cucumber Holothuria polii: Structural elucidation and stimulation of hematopoietic activity

The structural elucidation of polysaccharides is essential for understanding their structure-bioactivity relationship and related drug development. In this study, fucoidan (Fuc_hp) was extracted and purified from sea cucumber Holothuria polii. Its sulfate content was 39.5 ± 1.4%, and the "weight-average" molecular mass was 103.1 ± 2.8 kDa. The primary structure of Fuc_hp was clarified using a combination of acid degradation, tandem mass spectrometry, and nuclear magnetic resonance spectroscopy analysis. As a result, Fuc_hp was found to be composed of a tetrafucose repeating unit [→3-α-l-Fucp-1 → 3-α-l-Fucp2(OSO₃^-)-1 → 3-α-l-Fucp2(OSO₃^-)-1 → 3-α-l-Fucp2,4(OSO₃^-)-1→]. The stimulating hematopoiesis was further evaluated in a mouse model induced by cyclophosphamide. Based on these findings, intraperitoneally administered Fuc_hp may accelerate the recovery of white blood cells and neutrophils, in which its activity exceeded that of recombinant human granulocyte colony-stimulating factor (rhG-CSF). Meanwhile, in the background of cyclophosphamide-induced immunosuppression, treatment with Fuc_hp reduces platelet aggregation caused by CTX, so it might have the effect of reducing the risk of thrombosis. Therefore, Fuc_hp can be exploited as potentially promising stimulator of hematopoiesis in the future.