Enhanced bioactivity of Si₃N₄ through trench-patterning and back-filling with Bioglass®

Using a simple and innovative sandblasting process, disks of monolithic biomedical silicon nitride (β-Si₃N₄) were texturized with a matrix of regular, discrete square trenches with a total depth in the range of hundreds of microns. The process consisted of sandblasting Si₃N₄ substrates through a stainless-steel wire-mesh (150 or 200 μm) using abrasive silicon carbide powders (α-SiC, ∼40 μm) under 1,034 kPa (150 psi) of gas pressure. The depth of the porosities could be controlled varying both the treatment time and the distance from the surface. Part of the samples were then filled with 45S5 Bioglass® powders to improve the osteointegration and stimulate the production of bone tissue. Due to the increased macroscopic and microscopic roughness, biological testing using human osteosarcoma cells (SaOS-2) showed improved cell proliferation and greater production of both mineral (hydroxyapatite) and organic (collagen) phases on the patterned surfaces compared to untreated β-Si₃N₄ or to the biomedical titanium control samples. Both of these effects were further enhanced when the porosities were filled with Bioglass®.