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Long Non-Coding RNA CDKN2B-AS1 Facilitates Laryngeal Squamous Cell Cancer Through Regulating miR-497/CDK6 Pathway

Xiangyan Cui1, Tingting Yu1, Jing Shang1

  • 1Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Jilin University, Changchun 130021, People's Republic of China.

Oncotargets and Therapy
|November 23, 2019
PubMed
Abstract

Insights

CDKN2B-AS1 is upregulated in laryngeal squamous cell cancer (LSCC) and promotes tumor growth by regulating the miR-497/CDK6 axis. This long noncoding RNA may be a potential therapeutic target for LSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNA CDKN2B-AS1 (antisense RNA 1) is implicated in various cancers.
  • Its specific functions and regulatory mechanisms in laryngeal squamous cell cancer (LSCC) are not well understood.

Purpose of the Study:

  • To investigate the biological roles of CDKN2B-AS1 in LSCC.
  • To elucidate the underlying regulatory mechanisms of CDKN2B-AS1 in LSCC progression.

Main Methods:

  • Real-time quantitative PCR (qRT-PCR) to measure expression of CDKN2B-AS1, miR-497, and CDK6 in LSCC tissues and cell lines.
  • Functional assays to assess the impact of CDKN2B-AS1 on LSCC cell proliferation, apoptosis, migration, and invasion.
  • Bioinformatics analysis and luciferase activity assays to determine the interaction between CDKN2B-AS1 and miR-497.

Main Results:

  • CDKN2B-AS1 expression is significantly elevated in LSCC tissues compared to normal tissues.
  • High CDKN2B-AS1 levels correlate with lymph node metastasis and advanced clinical stage.
  • CDKN2B-AS1 knockdown inhibits LSCC cell proliferation, induces apoptosis, and suppresses migration and invasion.
  • CDKN2B-AS1 acts as an oncogenic lncRNA in LSCC by regulating the miR-497/CDK6 axis.

Conclusions:

  • CDKN2B-AS1 plays an oncogenic role in LSCC tumorigenesis via the miR-497/CDK6 pathway.
  • CDKN2B-AS1 represents a potential therapeutic target for LSCC treatment.
Keywords:
CDK6CDKN2B-AS1laryngeal squamous cell cancerlong noncoding RNAmiR-497

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