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Antibacterial Activity of Cissus incisa Extracts against Multidrug- Resistant Bacteria
Deyani Nocedo-Mena1,2, Elvira Garza-González3, Mauricio González-Ferrara4
1Faculty of Chemical Sciences, Autonomous University of Nuevo Leon, Av. Universidad S/N, Ciudad Universitaria, CP 66451, San Nicolas de los Garza, Nuevo Leon, Mexico.
Aims:
The need to find new antimicrobial agents to cope with this phenomenon increases.
Background:
Infection diseases are illness caused by different microorganisms, such as bacteria, among those caused by resistant bacteria are associated with greater morbidity, mortality and cost of the treatment than those caused by sensitive bacteria of the same species.
Objective:
Need to find new antimicrobial agents to cope with this phenomenon increases.
Methods:
This work carried out the study of biological activities of Cissus incisa, taking account its traditional use. Three extracts were prepared from the leaves of this plant: hexane, chloroform methanol (1:1) and aqueous. Their antibacterial and antitubercular activities were evaluated using microdilution and alamar blue assays; respectively.
Results:
The chloroform/methanol extract (1:1) was the most active of the three tested extracts for antimicrobial activity. In this way, the extract exhibits a broad spectrum of antimicrobial activity, against the Gram positive and Gram negative bacteria tested, with MIC values between 125 to 500 μg/mL.
Conclusion:
This research contributes both to the knowledge of the Mexican flora, as well as the discovery of potential antibacterial agents derivate from plants.
Insights
Researchers explored Cissus incisa for new antimicrobial agents. The chloroform/methanol extract showed broad-spectrum antibacterial activity, offering potential for novel drug discovery.
Area of Science:
- Ethnobotany and Phytochemistry
- Microbiology and Infectious Diseases
Background:
- Antimicrobial resistance is a growing global health threat, increasing morbidity, mortality, and treatment costs.
- Infectious diseases caused by resistant bacteria pose a significant challenge compared to those caused by sensitive strains.
Purpose of the Study:
- To investigate the biological activities of Cissus incisa, a plant with traditional medicinal uses.
- To identify potential new antimicrobial agents from plant-derived sources.
Main Methods:
- Preparation of three leaf extracts from Cissus incisa: hexane, chloroform/methanol (1:1), and aqueous.
- Evaluation of antibacterial and antitubercular activities using microdilution and alamar blue assays.
Main Results:
- The chloroform/methanol extract demonstrated the most potent antimicrobial activity among the tested extracts.
- This extract exhibited broad-spectrum activity against both Gram-positive and Gram-negative bacteria, with minimum inhibitory concentrations (MICs) ranging from 125 to 500 μg/mL.
Conclusions:
- This study contributes to the ethnobotanical knowledge of Mexican flora.
- Cissus incisa extracts show promise as a source of potential new antibacterial agents.
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