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Updated: Jul 13, 2026
Murine Model of Wound Healing
Published on: May 28, 2013
MMP-9 responsive dipeptide-tempted natural protein hydrogel-based wound dressings for accelerated healing action of
Jegatheeswaran Sonamuthu1, Yurong Cai1, Han Liu1
1The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
Abstract:
The infected diabetic wound ulcer is a significant problem for the diabetic patients, which leads to removal of affected foot site due to its delayed/non-healing tissues. The poly-microbial infections and active matrix metalloproteinases (MMP) are the significant influencing factors to delayed healing in diabetic mice. The main purposes of present investigation are to evaluation the targeted inactivation of MMP and avoid polymicrobial infections by using a combined therapeutic effect of metal chelating dipeptide (L-carnosine) and curcumin with the biocompatible silk protein hydrogel (L-car@cur/SF) dressing in the infected diabetic wound ulcer. The in vitro biological assay methods, such as, cell viability, anti-oxidant activity, anti-inflammatory macrophage cells and inhibition collagenase exhibited that the designed hydrogel matrix to be human cell compatible and could be accelerate for significant diabetic healing potential. The activation of cur/SF matrix by the L-carnosine was persuading the inactivation of matrix metalloproteinase-9 (MMP-9) through its potent chelating effects of Zn2+ ions from the MMP-9 active center. The L-car@cur/SF hydrogel was demonstrated for the effective MMP-9 inactivation and bacterial inhibition via in vivo mice wound site, which indorsed the diabetic wound healing efficiency in streptozotocin-tempted diabetic mice.
Insights
A novel hydrogel dressing combining L-carnosine and curcumin effectively treats infected diabetic wounds. This therapy inactivates matrix metalloproteinases (MMPs) and prevents infections, accelerating diabetic wound healing.
Area of Science:
- Biomaterials Science
- Wound Healing Research
- Diabetic Complications
Background:
- Diabetic foot ulcers present a significant clinical challenge, often leading to amputation due to delayed healing.
- Polymicrobial infections and elevated matrix metalloproteinases (MMPs) are key factors hindering wound closure in diabetic patients.
Purpose of the Study:
- To develop and evaluate a silk protein-based hydrogel (L-car@cur/SF) incorporating L-carnosine and curcumin for treating infected diabetic wounds.
- To assess the hydrogel's ability to inactivate MMPs and combat polymicrobial infections, thereby promoting wound healing.
Main Methods:
- In vitro assays including cell viability, antioxidant activity, anti-inflammatory effects on macrophages, and collagenase inhibition.
- In vivo studies using streptozotocin-induced diabetic mice to evaluate wound healing efficacy.
- Characterization of hydrogel matrix and its therapeutic mechanism involving L-carnosine's metal-chelating properties.
Main Results:
- The L-car@cur/SF hydrogel demonstrated human cell compatibility and accelerated diabetic wound healing potential in vitro.
- L-carnosine effectively inactivated matrix metalloproteinase-9 (MMP-9) by chelating Zn2+ ions from its active site.
- In vivo results showed significant MMP-9 inactivation, bacterial inhibition, and enhanced diabetic wound healing in mice.
Conclusions:
- The L-car@cur/SF hydrogel is a promising therapeutic agent for infected diabetic wounds.
- Targeted MMP inactivation and infection control are crucial for accelerating diabetic wound healing.
- This combined therapy offers a potential strategy to improve outcomes for diabetic patients with chronic wounds.
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