Fixation of CO₂, electron donor and redox microenvironment regulate succinic acid production in Citrobacter...

Biological sequestration of CO₂ for generating value added products is an emerging strategy. Succinic acid (SA) is an important C4 building block chemical, and its biological production via CO₂ sequestration, holds many practical applications. This study presents an in-depth insight on SA production using isolated strain belonging to genus Citrobacter, more closely related to Citrobacter amalonaticus by considering critical process parameters such as different carbon sources at various initial concentrations, buffering agent (NaHCO₃) concentrations and different pH conditions. The effect of H₂ gas as an electron donor and availability of CO₂ during SA production was also evaluated. The results from this work demonstrated that the isolated strain depicted the ability to utilize diverse carbon sources and highest SA production was achieved with sucrose as a substrate, indicating that reduced carbon substrates help in maximizing the redox potential. Incorporation of CO₂ and H₂ not only enhanced the production of SA but also affected the total acids profile favoring the production of SA over lactic, formic and acetic acids. Additional supply of CO₂ and H₂ led to maximum SA production of 12.07 gL^-1, productivity of 0.36 gL^-1 h^-1 and SA yield of 48.5%. In control operation when no gases were supplied and in other test conditions where either of the gases were supplied, lactic acid was the major end product followed by acetic acid. The positive effect of CO₂ for SA production provides scope for sustainable integration of SA and the CO₂-generating biofuel industries or industrial side streams.