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Generation of Multicellular Human Primary Endometrial Organoids
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Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent

Lior Pytowski1, Marek M Drozdz1,2, Haibo Jiang3

  • 1Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK.

International Journal of Molecular Sciences
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Summary

Reproductive hormones like oestrogen and progesterone induce the formation of the nucleoplasmic reticulum (NR) in endometrial cells. This process is reversible and offers a new model for studying NR in physiological conditions.

Keywords:
nuclear architecturenucleoplasmic reticulumreproductive cycle

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Area of Science:

  • Cell Biology
  • Reproductive Endocrinology
  • Nuclear Envelope Dynamics

Background:

  • The nuclear envelope can form invaginations, creating a nucleoplasmic reticulum (NR).
  • Extensive NR is linked to cellular senescence and some cancers, but its physiological formation is poorly understood.
  • Endometrial cells naturally exhibit significant nuclear invaginations.

Purpose of the Study:

  • To investigate the formation of the nucleoplasmic reticulum (NR) in endometrial cells under physiological conditions.
  • To determine the role of reproductive hormones in NR formation.
  • To establish a model for studying NR formation and function in a physiological context.

Main Methods:

  • Hormonal stimulation of endometrial cells with oestrogen and progesterone.
  • Microscopic observation of nuclear envelope invaginations and NR formation.
  • Analysis of incorporation of nascent lamins and phospholipids into NR.

Main Results:

  • Oestrogen and progesterone are sufficient to induce NR formation in endometrial cells.
  • NR formation is reversible upon removal of hormonal stimulus, without requiring cell division.
  • Nascent lamins and phospholipids are incorporated into the forming NR, suggesting specific machinery.

Conclusions:

  • Reproductive hormones play a key role in the physiological formation of the nucleoplasmic reticulum in endometrial cells.
  • NR formation in endometrial cells is a dynamic and reversible process.
  • This system provides a novel model for exploring the mechanisms and functions of NR in normal cellular conditions.