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Vision-Based Sensor for Three-Dimensional Vibrational Motion Detection in Biological Cell Injection.

Ferhat Sadak1, Mozafar Saadat1, Amir M Hajiyavand1

  • 1Department of Mechanical Engineering, School of Engineering, University of Birmingham, Birmingham B15 2TT, UK.

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Summary

This study introduces a non-invasive method using a microscope camera to measure injection pipette vibrations during intracytoplasmic sperm injection (ICSI). This visual sensing technique precisely monitors motion, improving control and identifying speed limitations for better infertility treatments.

Keywords:
3-D vibrationcomputer visionegg deformationimage processingintracytoplasmic sperm injection (ICSI)vision sensor

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Area of Science:

  • Biomedical Engineering
  • Reproductive Medicine
  • Optical Sensing

Background:

  • Intracytoplasmic sperm injection (ICSI) requires precise control of injection pipette motion.
  • Minimizing vibration in three axes is crucial for successful egg injection.
  • Physical vibration sensing is challenging due to practical limitations in ICSI procedures.

Purpose of the Study:

  • To propose and validate a non-invasive, vision-based technique for measuring 3D vibration displacement of the ICSI injection pipette.
  • To assess the impact of injection speed on lateral vibration and its relation to egg deformation and forces.

Main Methods:

  • Utilized a single microscope camera for non-invasive vibration measurement.
  • Employed contrast-limited adaptive histogram equalization (CHALE) and blob analysis for lateral and axial displacement.
  • Used the Brenner gradient algorithm for focus measurement.
  • Validated measurements against manual procedures across 4× to 40× magnifications.

Main Results:

  • Achieved measurement accuracy with differences up to 2% compared to manual methods.
  • Demonstrated that increased injection speed significantly elevates lateral vibration displacement by up to 54%.
  • Established a correlation between injection speed, lateral vibration, egg deformation, and penetration forces.

Conclusions:

  • Visual sensing provides a feasible and accurate method for measuring injection pipette vibrations in ICSI.
  • The developed technique can identify critical injection speed limits imposed by lateral vibrations.
  • This approach enhances precision and control in ICSI, potentially improving treatment outcomes.