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Analytical considerations for postmortem metabolomics using GC-high-resolution MS.

Lana Brockbals1, Thomas Kraemer1, Andrea E Steuer2

  • 1Department of Forensic Pharmacology and Toxicology, Zurich Institute of Forensic Medicine, University of Zurich, Winterthurerstrasse 190/52, 8057, Zurich, Switzerland.

Analytical and Bioanalytical Chemistry
|November 24, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a new gas chromatography-high-resolution mass spectrometry method for postmortem metabolomics. The validated method reliably assesses time-dependent changes in endogenous metabolites, aiding forensic investigations.

Keywords:
Forensic toxicologyGC-MSHigh resolutionMethod evaluationPostmortem metabolomics

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Area of Science:

  • Forensic Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Metabolomics is increasingly used in forensics for postmortem investigations.
  • Challenges like postmortem redistribution (PMR) remain unaddressed by current metabolomics methods.

Purpose of the Study:

  • Develop and validate an (un)targeted gas chromatography-high-resolution mass spectrometry (GC-HRMS) method for human postmortem metabolomics.
  • Establish a reliable tool for assessing time-dependent postmortem redistribution (PMR).

Main Methods:

  • Utilized a targeted metabolite subset (n=56) and a targeted processing workflow.
  • Employed an artificial matrix (revised simulated body fluid + BSA) for method calibration and validation.
  • Evaluated method performance including sensitivity, selectivity, accuracy, precision, and stability.

Main Results:

  • All analytical parameters met fitness-to-purpose criteria for reliability and robustness.
  • The method demonstrated suitability for large-scale postmortem metabolomics.
  • Recommendations include randomized sample processing for long runs and verification of biomarkers.

Conclusions:

  • The developed GC-HRMS method is suitable for time-dependent postmortem metabolomics investigations.
  • This tool can objectively assess postmortem redistribution (PMR).
  • The method provides a reliable foundation for future forensic metabolomics research.