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Immunoliposome-based fluorometric patulin assay by using immunomagnetic nanoparticles.

Xinjie Song1, Danhua Wang1, Myunghee Kim2

  • 1Department of Food Science and Technology, Yeungnam University, Gyeongsan-si, Gyeongsangbuk-do, 38541, Republic of Korea.

Mikrochimica Acta
|November 24, 2019
PubMed
Summary

A new fluorometric immunoassay accurately detects patulin, a toxic fungal metabolite, in apple juice. This rapid, eco-friendly method using immunomagnetic nanoparticles and immunoliposomes meets European safety limits for children's food.

Keywords:
Apple juice analysisFluorescence intensityHPLCPatulinRapid detection

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Food Safety

Background:

  • Patulin is a toxic fungal metabolite found in foods, posing health risks.
  • Accurate and rapid detection methods for patulin are crucial for food safety regulations.
  • Existing methods for patulin determination can be complex and time-consuming.

Purpose of the Study:

  • To develop a novel fluorometric immunoassay for the rapid and sensitive determination of patulin.
  • To create an assay utilizing immunomagnetic nanoparticles and immunoliposomes for efficient patulin capture and detection.
  • To validate the assay's performance in detecting patulin in apple juice, ensuring compliance with regulatory standards.

Main Methods:

  • Preparation of a rabbit anti-patulin-bovine serum albumin (BSA) IgG conjugate.
  • Development of immunoliposomes and immunomagnetic nanoparticles for patulin capture.
  • Formation of a sandwich complex involving patulin, antibodies, immunomagnetic nanoparticles, and immunoliposomes.
  • Magnetic separation of the complex followed by the release and fluorometric measurement of sulforhodamine B (SRB).

Main Results:

  • The assay successfully detected patulin in apple juice at concentrations as low as 8 μg L⁻¹.
  • The detection limit is within the European regulatory limit of 10 μg L⁻¹ for infant and children's food products.
  • The method eliminates the need for sample extraction, separation, and purification steps.
  • The assay demonstrated to be rapid, environmentally friendly, and reliable.

Conclusions:

  • The developed immunoliposome-based immunomagnetic nanoparticle assay provides a sensitive and efficient method for patulin determination.
  • This assay is suitable for routine food safety monitoring, particularly for apple juice and related products.
  • The method's simplicity and speed make it a valuable tool for ensuring compliance with food safety regulations.