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Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Published on: January 3, 2014
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A polerovirus, Potato leafroll virus, alters plant-vector interactions using three viral proteins
MacKenzie F Patton1, Aurélie Bak1, Jordan M Sayre2
1Department of Plant Pathology, University of California, Davis, California.
Plant, Cell & Environment
|November 24, 2019
Summary
Potato leafroll virus (PLRV) manipulates plant defenses, reducing jasmonic acid and ethylene. This viral manipulation enhances aphid attraction and reproduction on infected potato and tobacco plants.
Area of Science:
- Plant pathology
- Virology
- Insect-plant interactions
Background:
- Potato leafroll virus (PLRV) is a significant global potato pathogen.
- Aphids transmit PLRV, and infected plants attract and support higher aphid populations.
- The molecular basis for PLRV's effect on plant-aphid interactions is not well understood.
Purpose of the Study:
- To investigate the impact of PLRV infection on plant-aphid interactions and plant chemistry.
- To identify the molecular mechanisms by which PLRV influences plant defenses and aphid behavior.
Main Methods:
- Comparative analysis of plant-aphid interactions and chemical profiles in infected and control plants (Solanum tuberosum and Nicotiana benthamiana).
- Measurement of aphid-induced jasmonic acid (JA) and ethylene levels.
- Transient expression of PLRV proteins (P0, P1, P7) in Nicotiana benthamiana, followed by insect bioassays and chemical analyses.
Main Results:
- PLRV infection significantly attenuates the induction of aphid-induced jasmonic acid and ethylene in both potato and tobacco.
- Expression of PLRV proteins P0, P1, and P7 in Nicotiana benthamiana alters plant-aphid interactions.
- These PLRV proteins inhibit aphid-induced jasmonic acid and ethylene production.
Conclusions:
- PLRV actively manipulates plant defense pathways (JA and ethylene) to enhance its transmission efficiency.
- Specific PLRV proteins (P0, P1, P7) are key mediators of this manipulation, affecting plant chemistry and aphid behavior.
- This research reveals novel mechanisms of plant virus manipulation at the plant-vector-pathogen interface.
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