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Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
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Assessing equine embryo developmental competency by time-lapse image analysis.

Kelsey E Brooks1, Brittany L Daughtry2, Elizabeth Metcalf3

  • 1Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, USA.

Reproduction, Fertility, and Development
|November 25, 2019
PubMed
Summary
This summary is machine-generated.

Early equine embryo development shows significant chromosomal missegregation, impacting blastocyst formation. Time-lapse imaging offers a non-invasive method to assess equine embryo quality and mitotic timing.

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Mitotic timing is crucial for mammalian preimplantation embryo development.
  • Previous research has not closely examined mitotic timing in equine embryos derived from ovum pick-up procedures.

Purpose of the Study:

  • To investigate the timing of early mitotic events in equine embryos.
  • To assess equine embryo quality using time-lapse imaging and identify potential developmental issues.

Main Methods:

  • Cumulus-oocyte complexes were collected via transvaginal follicular aspiration, matured in vitro, and fertilized using intracytoplasmic sperm injection.
  • Fertilized oocytes were cultured to the blastocyst stage and monitored using time-lapse imaging to measure cell cycle intervals.
  • Morphological criteria and chromosomal integrity were assessed to evaluate developmental potential.

Main Results:

  • Of 56 fertilized oocytes, 35 initiated mitosis and 11 reached the blastocyst stage.
  • Typical blastocyst timing for the first three mitotic divisions was determined (median±IQR): 30.0±17.5min, 8.8±1.7h, and 0.6±1.4h.
  • High incidence of cellular fragmentation, multipolar divisions, and chromosomal abnormalities (micronuclei, multinuclei, DNA damage) were observed, suggesting frequent missegregation.

Conclusions:

  • Equine embryos exhibit significant chromosomal missegregation during preimplantation development.
  • Time-lapse imaging analysis is a viable non-invasive method for assessing equine embryo quality and mitotic timing.