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Updated: Jan 3, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Serendipitous discovery of aryl boronic acids as β-lactamase inhibitors
Shu-Wei Yang1, Jianping Pan1, Yuriko Root1
1Discovery Chemistry, Merck & Co., Inc., Kenilworth, NJ 07033, USA.
Abstract:
High throughput screening for β-lactamase inhibitors afforded biphenyl hits such as 1. Hit confirmation and X-ray soaking experiments with Pseudomonas Aeruginosa AmpC enzyme led to the identification of an aryl boronic acid-serine complex 4, which was formed from phenyl boronic acid 8 (an impurity in compound 1) and ethylene glycol (the cryoprotectant in the soaking experiment).
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