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A new "turn-on" fluorescence probe based on hydrazine-triggered tandem reaction
Wenzhi Xu1, Xue Li1, Mengnan Han1
1Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
Abstract:
Hydrazine is extremely harmful to the human body. The leakage of hydrazine is liable to cause potential safety hazards. Here, we reported a new fluorescence probe based on the tandem reaction. The hydrazine-triggered hydrazinolysis-cyclization resulted in the formation of the iminocoumarin. The fluorescence intensity at 522 nm of the probe increased after the reaction with hydrazine. There was a linear relationship between the fluorescence intensity and the concentration of hydrazine (0.14-120.00 μM). The LOD of the probe to N2H4 was 1.36 ppb. Notably, the probe could detect hydrazine in BT474 cells and tap water.
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