Does jasmonic acid regulate photosynthesis, clastogenecity, and phytochelatins in Brassica juncea L. in response to...

The present experiment unravels how exogenous jasmonic acid regulates photosynthesis, clastogenecity, AsA-GSH cycle and phytochelatins in Brassica juncea L. in response to Pb-subcellular distribution. The plants were evaluated for leaf gas exchange parameters, Fv/Fm, lipid peroxidation, leaf epidermal structures and ABA content. Besides lead accumulation in root, shoot and its subcellular distribution pattern, its role as clastogen and/or aneuploidogen via DNA damage, genome size and ploidy variations, AsA-GSH cycle and quantification of PC₂ and PC₃ were performed as well. Results revealed that Pb inhibited plant growth, disturbed epidermal and guard cells and consequently worsen leaf gas exchange parameters (E, GH₂O, A), F_v/F_m and photosynthetic pigments. For clastogenecity, results revealed considerable DNA damage and analysis for genome size showed that differences between unstressed, Pb-stress and JA application were not significant (P ≤ 0.05), however, ploidy ratio analysis proved partial aneuploidogenic role of Pb. The highest Pb exposure affected AsA-GSH cycle negatively but increased PC₂ and PC₃ contents uniformly in roots and leaves. Surprisingly, exogenous JA inhibits plant growth under non-stress but positively regulates growth, photosynthesis, AsA-GSH cycle, PC₂ and PC₃ contents and DNA damage but has no significant effect on variations in total genome size and ploidy under Pb-stress.