Differentiation and targeting of HT 29 cancer cells based on folate bioreceptor using cysteamine functionalized gold...

Cancer is one of the main causes of death worldwide. To decrease the mortality of cancer, early stage detection of cancer is of great importance. An innovative platform was developed for differentiation and detection of HT 29 cancer cells based on interactions between folate (FA) and folate receptors (FRs) of the membrane of cancer cells. In summary, FA and cysteamine (CA)-functionalized gold nanoparticles (AuNPs) were synthesized and characterized by field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier-transform infrared (IR) spectroscopy. Also, the surface charge was determined by measuring of the zeta potential. Fluorescence imaging and flow cytometry analyses were used to approve the selective uptake of the synthesized probe to the cancer cells. HEK 293 FR-negative cells were applied to assess the selectivity of AuNPs/CA/FA towards FR-negative cells. The differential pulse voltammetry (DPV) technique was used to determine the HT 29 cells from 250 to 5000 cells/mL with a lower limit of quantification (LLOQ) of 250 cells/mL. The produced AuNPs/CA/FA based nanoprobe could not only detect the signaling of HT 29 cells but also improve the specificity of cytosensor towards FR-positive cancer cells. According to the obtained results, the newly developed nano-probe could be used as a portable biomedical device for cancer diagnosis.