Malonyl-based Chemosensors: Selective Detection of Fe³⁺ Ion in Aqueous Medium

Two novel malonyl-based chemosensors, N,N'-bis(ethyl-4'-benzoate)-1,3-propanediamide (1) and N,N'-bis(ethyl-3'-benzoate)-1,3-propanediamide (2), have been synthesized and screened towards various biologically important metal ions such as Na⁺, Mg²⁺, K⁺, Ca²⁺, Al³⁺, Cr³⁺, Mn²⁺, Fe²⁺, Fe³⁺, Co²⁺, Ni²⁺, Cu²⁺, Zn²⁺, Ag⁺, Cd²⁺, Hg²⁺, Ti³⁺, and Pb²⁺. The emission spectral studies of both 1 and 2 displayed 84 - 91% turn-off emission responses selectively with Fe³⁺ ion in aqueous buffer (MeCN/H₂O, 1:4, v/v, pH = 7.4) solution. Chemosensors 1 and 2 exhibited remarkable sensing ability towards Fe³⁺ ion over other metal ions with limit of detection (LOD) of 4.28 and 4.33 μM, respectively. The binding stoichiometry of 1 and 2 with Fe³⁺ ion was studied by Benesi-Hildebrand fitting, Stern-Volmer plot and Job's plots, revealing that both chemosensors (1 - 2) bind with Fe³⁺ metal ion in 1:1 stoichiometric ratio with the apparent association constant (K_a) 8.90 × 10³ and 11.16 × 10³ M^-1, respectively. Furthermore, the interactions of chemosensors (1 - 2) with metal ion were also investigated by using density functional theory (DFT) at B3LYP hybrid functional using 6-31G and LanL2DZ basis sets.