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IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
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A Novel Ratiometric Cationic Iridium(III) Complex Phosphorescent Probe for Hydrogen Peroxide.

Xuanxian Wu1, Qi Lin2

  • 1College of Ocean, Minjiang University, Fuzhou, 350116, China.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|November 26, 2019
PubMed
Summary

A new iridium(III) complex probe (Ir-BE) detects hydrogen peroxide (H₂O₂) with a color change. This highly selective probe offers accurate, low-level H₂O₂ quantification in real water samples.

Keywords:
Iridium complexStokes shifthydrogen peroxideratiometric probe

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Inorganic Chemistry

Background:

  • Hydrogen peroxide (H₂O₂) is a crucial reactive oxygen species involved in various biological processes and environmental phenomena.
  • Accurate and selective detection of H₂O₂ is vital for understanding its roles and for environmental monitoring.
  • Existing detection methods often face limitations in selectivity, sensitivity, or operational conditions.

Purpose of the Study:

  • To design and synthesize a novel cationic iridium(III) complex phosphorescent probe (Ir-BE) for H₂O₂ detection.
  • To investigate the photophysical properties and sensing performance of the Ir-BE probe.
  • To evaluate the probe's applicability in real water samples.

Main Methods:

  • Synthesis of a cationic iridium(III) complex incorporating an aromatic boronate ester recognition unit.
  • Characterization of the probe's photoluminescent properties, including emission spectra and Stokes shift.
  • Evaluation of the probe's response to H₂O₂ across a broad pH range (4-13).
  • Quantitative analysis of H₂O₂ concentrations using ratiometric emission changes and determination of detection limits.

Main Results:

  • The synthesized Ir-BE probe exhibited ratiometric emission variations (I₄₉₀/I₅₅₀) with a distinct color change from yellow to green in the presence of H₂O₂.
  • The probe demonstrated a broad pH working range (4-13) and a large Stokes shift (320 nm).
  • A strong linear correlation (R² = 0.998) was observed between the emission ratio and H₂O₂ concentration (0-300 μM), with a low detection limit of 0.21 μM.
  • The Ir-BE probe showed excellent selectivity for H₂O₂ and was successfully applied for its quantitative detection in real water samples.

Conclusions:

  • The novel Ir-BE probe offers a sensitive, selective, and robust platform for the ratiometric detection of H₂O₂.
  • Its broad pH tolerance and large Stokes shift make it advantageous over existing probes.
  • The probe's successful application in real water samples highlights its potential for practical environmental and biological monitoring.