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Updated: Jul 31, 2026
Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
Published on: July 4, 2018
In vitro assessment of metabolic profile of Enterococcus strains of human origin
Ashlesha Bhagwat1, Uday S Annapure2
1Department of Food Engineering and Technology, Institute of Chemical Technology, Mumbai, 400019, India.
Background:
In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019).
Results:
Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5-0.7 mg ml-1), protease (192-264 mg ml-1), lipase (8-10 mg ml-1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml-1), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 °C. Castor oil (highest yield 60 μg ml-1) and sunflower oil (highest yield 48 μg ml-1) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30-50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains.
Conclusion:
The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.
