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Related Experiment Video

Updated: Jan 3, 2026

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Published on: November 7, 2019

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids.

Daehan Kim¹, Gi-Hun Lee¹, Jiheum Park²

¹Department of Mechanical Engineering, Graduate School, Chung-Ang University.

Journal of Visualized Experiments : Jove

|November 26, 2019

Summary

This summary is machine-generated.

A novel centrifugal microfluidic-based spheroid (CMS) culture system enables the creation of 3D cell spheroids using high centrifugal force. This lab-on-a-CD platform facilitates advanced cell biology and tissue engineering research.

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Last Updated: Jan 3, 2026

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

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Published on: November 7, 2019

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Published on: August 9, 2024

Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

Three-dimensional (3D) cell cultures better mimic in vivo microenvironments than 2D cultures.
3D spheroids are crucial for accurate cell-based experiments and disease modeling.
Existing methods for spheroid generation can be complex or lack control over microenvironment simulation.

Purpose of the Study:

To develop an automated, centrifugal microfluidic platform for generating 3D cell spheroids.
To create a lab-on-a-CD system capable of simulating high-gravity conditions for cell culture.
To demonstrate the system's utility in producing diverse spheroid structures for research.

Main Methods:

Fabrication of a centrifugal microfluidic-based spheroid (CMS) culture system on a compact disc using polydimethylsiloxane and a polycarbonate mold.
Implementation of an electrical motor to drive the CD platform, generating variable centrifugal forces (1-521 x g).
Utilizing a barrier wall and slide region within the microfluidic channels for even cell distribution and entry into 400 μm microwells.

Main Results:

The CMS system successfully generated 3D cell spheroids under high gravity conditions.
Monoculture and coculture spheroids were produced using human adipose-derived stem cells and human lung fibroblasts.
The system facilitated the creation of various coculture spheroid structures, including concentric, Janus, and sandwich types.

Conclusions:

The centrifugal microfluidic-based spheroid (CMS) culture system offers a simple yet effective method for 3D spheroid generation.
This technology enhances cell culture capabilities, providing a valuable tool for cell biology and tissue engineering.
The CMS system supports diverse spheroid culture applications, including single and multiple cell types for organoid development.