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Development of response surface methodology for optimization of parameters and quantitative analysis of chebulinic
T Mohammad Munawar1, D V Surya Prakash2, Meena Vangalapati2
1Department of Biotechnology, JNTUA College of Engineering, Pulivendula 516390, Andhra Pradesh, India.
Abstract:
The purpose of the research is to study the development of response surface methodology for optimization of chebulinic acid extraction from composition of medicinal herbs such as Terminalia chebula, Phyllanthus emblica and seeds of Dimocarpus longan. Optimization of extraction parameters such as weight dosages, pH and time were carried out by response surface methodology (RSM). The optimal conditions determined for extraction of chebulinic acid through response surface methodology were dosage (6.25 g), pH (5.7) and time (24.23 h). These results showed that the developed model is satisfactory and relevant for the extraction of chebulinic acid. The analysis of variance showed a high goodness of model fit and the performance of the RSM method for improving chebulinic acid extraction from the composition of medicinal herbs. Quantitative estimation of chebulinic acid in the composition of medicinal herbs by HPLC studies revealed that 0.712% w/w of chebulinic acid content was present in the composition of herbal powder.
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