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Alteration in MDA, GSH level and hematological changes due to thiamine deficiency in Mus musculus
1Bioscience and Biotechnology Department, Banasthali University, Banasthali-304022, Rajasthan, India.
Abstract:
It is known that thiamine deficiency may lead to Alzheimer's diseases in humans. The present study has thus been conducted to understand the role of thiamine deficiency with respect to alteration in the peripheral blood of Swiss albino mice. For this purpose, adult Swiss albino mice (6-8 week old) were divided into three groups. The first group was control; the second (group II) and the third group (group III) were made thiamine deficient for 08 and 10 days respectively. Thiamine deficiency was induced in mice by injecting pyrithiamine (5 µg/10 g bwt) and feeding a thiamine deficient diet. The erythrocytes, leukocytes count, hemoglobin, hematocrit value, mass cell volume, mean corpuscular hemoglobin in blood of mice were determined by hematoanalyzer. Malondialdehyde (MDA) and reduced glutathione (GSH) level was also determined in serum of treated and non-treated groups. A significant reduction in leukocyte and erythrocyte count was observed in both the thiamine deficient groups as compared to control. Levels of hemoglobin and hematocrit value were also declined in the thiamine deficient groups. Enhancement in mass cell volume (MCV) level and decline in mean corpuscular hemoglobin (MCH) levels were observed in both thiamine deficient groups with respect to control. Inter-group comparison of all parameters also showed a significant value at p<0.01. In comparison with the control group, elevation in MDA and decline in GSH level was observed in both thiamine deficient groups which were statistically significant. These data indicate that thiamine deficiency leads to significant alterations in the hematological parameters as well as in MDA and GSH level.
Insights
Thiamine deficiency significantly alters peripheral blood in mice, impacting red and white blood cell counts, hemoglobin, and hematocrit. It also affects oxidative stress markers, increasing malondialdehyde and decreasing glutathione.
Area of Science:
- Biochemistry
- Hematology
- Neuroscience
Background:
- Thiamine deficiency is linked to neurological disorders like Alzheimer's disease.
- Understanding its effects on peripheral blood is crucial for disease research.
Purpose of the Study:
- To investigate the impact of thiamine deficiency on hematological parameters and oxidative stress in Swiss albino mice.
- To establish a model for studying thiamine deficiency-related blood alterations.
Main Methods:
- Induction of thiamine deficiency in mice using pyrithiamine injection and a deficient diet for 8 and 10 days.
- Analysis of blood parameters including erythrocyte count, leukocyte count, hemoglobin, hematocrit, MCV, and MCH using a hematoanalyzer.
- Measurement of serum malondialdehyde (MDA) and reduced glutathione (GSH) levels.
Main Results:
- Significant reductions in leukocyte and erythrocyte counts, hemoglobin, and hematocrit were observed in thiamine-deficient groups.
- Increased mean corpuscular volume (MCV) and decreased mean corpuscular hemoglobin (MCH) were noted.
- Elevated MDA and decreased GSH levels indicated increased oxidative stress in thiamine-deficient mice.
Conclusions:
- Thiamine deficiency causes significant hematological changes in mice.
- The deficiency also leads to oxidative stress, evidenced by altered MDA and GSH levels.
- These findings highlight the systemic impact of thiamine deficiency beyond neurological effects.
