Characterization of the phage-specific transfer RNA molecules coded by cholera phage phi 149

Aminoacylation of tRNA isolated from choleraphage phi 149-infected cells with individual 3H-labeled L-amino acids followed by hybridization with phage DNA revealed that the phage encodes tRNAs specific for arginine, proline, glycine, isoleucine, serine, valine, tyrosine, histidine, lysine, leucine, tryptophan, and aspartic acid. Aminoacylation of phage-coded tRNAs isolated from phage DNA-RNA hybrids also confirmed this observation except for tryptophan.