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Rift Valley Fever Virus: Propagation, Quantification, and Storage
MaRyka R Smith1, Erin E Schirtzinger2, William C Wilson2
1Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas.
Current Protocols in Microbiology
|November 26, 2019
Summary
Rift Valley fever virus (RVFV) causes severe disease in livestock and humans. This article details laboratory methods for propagating and quantifying RVFV, crucial for developing effective diagnostics and vaccines.
Area of Science:
- * Virology
- * Molecular Biology
- * Public Health
Background:
- * Rift Valley fever virus (RVFV) is a zoonotic, arthropod-borne pathogen endemic to Africa and the Arabian Peninsula.
- * RVFV outbreaks cause high mortality in livestock and severe hepatitis in humans.
- * Current vaccines are limited, necessitating robust laboratory research methods.
Purpose of the Study:
- * To present reliable laboratory techniques for RVFV propagation and quantification.
- * To provide protocols for researchers working with RVFV in vitro.
- * To support the development of diagnostics and therapeutics for Rift Valley fever.
Main Methods:
- * Propagation of RVFV in mammalian and insect cell cultures.
- * Quantification using plaque assays, TCID50 assays, and focus-forming assays.
- * Protocols for virus storage, disinfection, and titer calculation.
Main Results:
- * Established and validated methods for RVFV propagation in various cell types.
- * Demonstrated successful quantification of infectious RVFV particles.
- * Provided standardized protocols for consistent research outcomes.
Conclusions:
- * The presented methods are essential for routine RVFV research.
- * These techniques facilitate the study of RVFV replication and pathogenesis.
- * Standardized protocols are critical for advancing RVFV vaccine and therapeutic development.
