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Updated: Jan 3, 2026

Microiontophoresis and Micromanipulation for Intravital Fluorescence Imaging of the Microcirculation

Microiontophoresis and Micromanipulation for Intravital Fluorescence Imaging of the Microcirculation

Published on: June 10, 2011

The diffusive injection micropipette (DIMP).

Alexander H Howell¹, Winfried S Peters¹, Michael Knoblauch¹

¹School of Biological Sciences, Washington State University, Pullman, WA, 99164, USA.

Journal of Plant Physiology

|November 26, 2019

Summary

This summary is machine-generated.

The diffusive injection micropipette (DIMP) offers a less invasive method for delivering substances into live cells. This novel technique minimizes cellular disruption and artifacts compared to traditional microinjection methods.

Keywords:

Cytoplasmic streaming Fluorescent probe Fluorophore Microinjection Nicotiana Trichome

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Area of Science:

Cell Biology
Biotechnology
Microscopy

Background:

Microinjection is crucial for cell biology research.
Conventional methods can cause cellular artifacts and interfere with function.

Purpose of the Study:

To develop a less invasive microinjection technique.
To reduce artifacts associated with cargo delivery into live cells.

Main Methods:

Design and implementation of the diffusive injection micropipette (DIMP).
Testing DIMP in plant, fungal, and animal cells.
Assessing non-invasiveness by observing cytoplasmic dynamics post-impalement.

Main Results:

DIMP significantly reduced cellular disruption compared to pressure microinjection.
Successful cargo delivery into diverse cell types.
DIMP minimizes artifacts inherent in other microinjection techniques.

Conclusions:

DIMP is a cost-effective and easy-to-produce tool for minimally invasive cellular delivery.
DIMP offers a superior alternative for live-cell studies requiring precise material introduction.
This technique has broad applicability across various cell types.