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Genistein Modulates Signaling Pathways and Targets Several Epigenetic Markers in HeLa Cells

Madhumitha Kedhari Sundaram1, Sreepoorna Unni2, Pallavi Somvanshi3

  • 1School of Life Sciences, Manipal Academy of Higher Education, P.O. Box 345050 Dubai, United Arab Emirates.

Genes
|November 27, 2019
PubMed
Abstract

Insights

Genistein, a phytochemical, reverses epigenetic changes and restores tumor suppressor gene expression in HeLa cells. This suggests genistein

Area of Science:

  • Epigenetics and Molecular Oncology
  • Phytochemicals and Cancer Therapeutics

Background:

  • Epigenetic alterations drive carcinogenesis by modifying signaling pathways and tumor suppressor genes (TSGs).
  • HeLa cells are utilized to investigate the molecular effects of genistein, a plant-derived compound.

Purpose of the Study:

  • To investigate the impact of genistein on molecular targets involved in cancer.
  • To elucidate the epigenetic mechanisms underlying genistein's anti-cancer effects.

Main Methods:

  • Quantitative PCR (qPCR) for gene expression analysis.
  • Biochemical assays for epigenetic enzyme activity.
  • Quantitative methylation array and methylation-specific restriction digestion for promoter methylation analysis.

Main Results:

  • Genistein modulated genes in cell cycle, migration, inflammation, PI3K, and MAPK pathways.
  • Genistein altered the expression and activity of epigenetic modifiers, including DNA methyltransferases (DNMTs) and histone deacetylases (HDACs).
  • Genistein reduced global DNA methylation and specific TSG promoter methylation, leading to increased gene transcription.

Conclusions:

  • Genistein exhibits anti-cancer potential through comprehensive epigenetic modulation and transcriptional restoration of TSGs.
  • The findings support genistein's development as a therapeutic candidate for cancer treatment.
Keywords:
cancerepigeneticgenisteintumour suppressor gene

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