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microRNAs Regulating Human and Mouse Naïve Pluripotency.

Yuliang Wang1,2, Abdiasis M Hussein2,3, Logeshwaran Somasundaram2,3

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|November 27, 2019
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MicroRNAs regulate key cell state transitions like pluripotency and embryonic diapause. This study identifies conserved microRNAs and targets crucial for these developmental stages in humans and mice.

Keywords:
embryonic diapausemicroRNAnaïve and primed pluripotent stem cellsshh

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Area of Science:

  • Developmental Biology
  • Epigenetics
  • Molecular Biology

Background:

  • MicroRNAs (miRNAs) are non-coding RNAs regulating gene expression post-transcriptionally.
  • miRNAs are critical for cell fate decisions, including pluripotency transitions and embryonic diapause.
  • The role of miRNAs in human and mouse pluripotency and diapause requires further exploration.

Purpose of the Study:

  • To identify conserved microRNAs and their target genes involved in naïve to primed pluripotency transitions.
  • To investigate microRNAs and target genes associated with the embryonic diapause state.
  • To provide a comprehensive resource for miRNA regulation in early development.

Main Methods:

  • Meta-analysis of microRNA-seq, RNA-seq, and metabolomics datasets from human and mouse.
  • Identification of microRNAs and experimentally validated target genes with consistent expression changes.
  • Analysis of regulated pathways, including developmental signaling, primary cilia, and metabolic processes.

Main Results:

  • Identified conserved microRNAs and target genes showing reciprocal expression changes during naïve to primed pluripotency transitions.
  • Found 115 microRNAs consistently altered in human and mouse pluripotency transitions, including novel candidates.
  • Discovered 38 microRNAs and 274 target genes potentially involved in embryonic diapause, suggesting miRNA-mediated stress response activation.

Conclusions:

  • This study provides a comprehensive resource of microRNAs and their targets regulating pluripotency and diapause.
  • Conserved microRNA regulatory networks are critical for key developmental transitions in mammalian embryos.
  • Identified novel microRNAs as potential regulators of pluripotency and embryonic developmental arrest.