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Mannheimia haemolytica IgA-specific proteases.

Sahlu Ayalew1, Betsy K Murdock1, Timothy A Snider1

  • 1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, 74078-2007, USA.

Veterinary Microbiology
|November 27, 2019
PubMed
Summary

Mannheimia haemolytica produces two immunogenic IgA proteases that cleave bovine IgA, aiding colonization. Antibodies against these proteases are found in cattle recovering from respiratory disease.

Keywords:
AutotransporterBovine respiratory diseaseIgA proteaseMannheimia haemolytica

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Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Immunology

Background:

  • Mannheimia haemolytica causes bovine pleuropneumonia.
  • Bacterial IgA proteases cleave immunoglobulin A (IgA), facilitating mucosal colonization.

Purpose of the Study:

  • Characterize M. haemolytica IgA1 and IgA2 proteases.
  • Clone and sequence the genes encoding these proteases.
  • Assess the immunogenicity of IgA protease components.

Main Methods:

  • Gene cloning, expression, and sequencing.
  • In vitro cleavage assays of bovine IgA.
  • In silico sequence analysis.
  • Immunization of mice and analysis of antibody responses.
  • Serological examination of cattle sera.

Main Results:

  • Two IgA protease genes were successfully cloned, expressed, and sequenced.
  • M. haemolytica culture supernatant cleaved purified bovine IgA.
  • Both IgA proteases possess IgA peptidase, PL1_Passenger_AT, and autotransporter domains.
  • Convalescent cattle sera showed high antibody titers against IgA protease components.
  • Mice immunized with protease components developed antibodies against IgA peptidases and autotransporters.

Conclusions:

  • M. haemolytica produces two immunogenic IgA proteases that cleave bovine IgA.
  • These proteases are produced in vivo, as indicated by antibodies in convalescent cattle.
  • Further research is warranted on the role of IgA proteases in M. haemolytica pathogenesis and immunity.