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Related Experiment Video

Updated: Jan 3, 2026

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

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3D printed microfluidic devices for circulating tumor cells (CTCs) isolation.

Juhong Chen1, Chun-Yen Liu2, Xinchang Wang3

  • 1Department of Mechanical Engineering, University of California, Berkeley, USA; Berkeley Sensor and Actuators Center, USA; Department of Biological Systems Engineering, Virginia Tech, USA; Hangzhou Institute of Advanced Transducing Technology, Wahaha R&D Academe, Hangzhou, China.

Biosensors & Bioelectronics
|November 27, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a novel 3D printed microfluidic device for isolating circulating tumor cells (CTCs) from blood. The device achieves over 90% capture efficiency, aiding cancer diagnosis and treatment strategies.

Keywords:
3D printingCirculating tumor cell (CTC)DiagnosticsImmunocaptureMicrofluidic separation

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Area of Science:

  • Biomedical Engineering
  • Oncology
  • Microfluidics

Background:

  • Isolating circulating tumor cells (CTCs) is crucial for cancer prognosis and therapy.
  • Low CTC concentration in blood presents significant isolation challenges.
  • EpCAM (epithelial cell adhesion molecule) is a key biomarker for many cancer types.

Purpose of the Study:

  • To develop and validate a novel 3D printed microfluidic device for efficient CTC isolation.
  • To functionalize the device with anti-EpCAM antibodies for targeted tumor cell capture.
  • To assess the device's performance in capturing various cancer cell lines and from human blood samples.

Main Methods:

  • Fabrication of a microfluidic device using 3D printing technology with optimized interior structures.
  • Functionalization of the device surface with anti-EpCAM antibodies.
  • Testing the device with EpCAM-positive (MCF-7, SW480, PC3) and EpCAM-negative (293T) cancer cell lines at an optimal flow rate of 1 mL/h and channel length of 2 cm.
  • Demonstration of MCF-7 cell isolation from spiked human blood samples.

Main Results:

  • The 3D printed microfluidic device demonstrated high surface area and effective fluid flow manipulation.
  • Achieved a capture efficiency exceeding 90% for EpCAM-positive cancer cell lines.
  • Successfully isolated MCF-7 breast cancer cells from spiked human blood samples.
  • Validated the device's potential for isolating CTCs from patient blood.

Conclusions:

  • The novel 3D printed microfluidic device offers a highly efficient method for CTC isolation.
  • The device shows promise for improving cancer diagnostics and therapeutic monitoring.
  • Future directions include combining CTC capture with DNA-based analysis for comprehensive cancer assessment.