Mitochondria-targeting turn-on fluorescent probe for HClO detection and imaging in living cells

Hypochlorous/hypochlorite (HClO/ClO^-), one of the most important signal molecule, plays a crucial role in many cellular signaling pathways. It is reported that the HClO/ClO^- level in mitochondria is important to maintain the normal mitochondrial function. Herein, we present two simple fluorescent probes BAC and mitochondria-targeting fluorescent probe TACB for the detection of ClO^-. Probes BAC &TACB could be sensitively and selectivity detecting ClO^- at the nanomolar levels with the detection limit of 1.64 × 10^-9 M and 9.86 × 10^-8 M, respectively. Additionally, probes BAC &TACB with the response unit of CO moiety could selectively detect ClO^- over other various analytes such as anions, metal ions and OH, ¹O₂, H₂O₂. The response time of probe TACB for ClO^- (<20 s), implying that it could offer a real-time analytical assay of ClO^-. Finally, probe BAC was used for monitoring the ClO^- in HEK293T cells and probe TACB could be utilized to track the fluctuations of exogenous ClO^- levels in the mitochondria of Hela cells.